Our cell culture facility includes the infrastructure and equipment
to support research for cell and tissue culture test systems.
  • Class II biological safety cabinets
  • Multiple CO2, relative humidity and temperature regulated incubators
  • Variety of centrifuges
  • Variety of freezers and refrigerators

Characterization Equipment

  • Microscopy
    • Bright Field
    • Phase Contrast
    • Fluorescence
    • Electron
  • Spectroscopy
    • Absorbance
    • Fluorescence
  • Other Diagnostics
    • RT-PCR
    • Microarray Scanner
medical device coating machine

Current in vitro Cell Culture Work with Human Cells

Primary Cells

  • Astrocyte
  • Cardiac Myocyte
  • Chondrocyte
  • Endothelial
  • Epithelial
  • Fibroblast
  • Hepatocyte
  • Macrophage
  • Mesenchymal Stem
  • Neural Stem
  • Neuron
  • Osteoblast
  • Schwann Cells
  • Skeletal
  • Smooth Muscle
  • Urothelial
  • And More
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Cellular Assays

Binding Adhesion/Efficiency/Proliferation

Various cell-type-material combinations can be qualitatively and quantitatively evaluated for cell
adhesion and proliferation behavior over an incubation period between 1 and 28 days.

  • Cell Count – Live and/or DEAD – Nuclear and Cytoplasmic Staining
  • DNA Quantification
  • Cell-to-Cell and/or Cell-to-Surface Adhesion
Osteoblast and fibroblast cell attachment on adhesion platforms for peek implant surface treatments
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Cellular Structural Integrity/Metabolic Activity - Cytotoxicity

Cytotoxicity screening assays can be used to evaluate the response of living cells to
material surfaces.

  • MTT ((3-(4,5-dimethylthiazol)-2-yl)-2,5-diphenyltetrazolium bromide)
  • LDH (Lactate Dehydrogenase)
  • NRU (Neutral Red Uptake)
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Intracellular and Extracellular - Visualization

Static and dynamic morphological responses to various substrate surfaces can be
qualitatively and quantitatively analyzed – size, spreading, population and organization.
  • Immunofluorescence Imaging
  • Label-Free Imaging (Scanning Electron Microscopy/SEM)

Differentiation and Markers

Cells seeded on different surfaces can be evaluated for specific gene, protein or molecular
markers over the incubation period (e.g., bone matrix formation).

Cell Proliferation

Procollagen I, Transforming Growth Factor-beta (TGF-b), and/or Fibronectin

Matrix Maturation
Alkaline Phosphatase (ALP)
Matrix Mineralization
Osteocalcin (OC), Osteopontin (OP), Bone Sialoprotein (BSP) and/or Calcium
dental implant surface treatment
biocompatible coating for implants

Development

Co-Culture/Competitive and 3D Bioassays
In addition to being equipped to perform a number of standard cell-based bioassays, we have the
ability to develop standard and specialized co-culture and 3D culture systems.
  • Materials/reagents and equipment to formulate and optimize specialized scaffolds and models
  • Equipment and materials to coat or modify the surface of specialized scaffolds
  • Equipment for imaging and analyzing cell-cell interaction, cell proliferation, population, gene and protein expression, toxicity, etc.

Surface Characterization Capabilities

Surface Characterization

  • Surface morphology (SEM)
  • Surface roughness (AFM)
  • Surface energy (contact angle)

Protein Binding

  • Total protein assay – colorimetric assay technique

Simulated Body Fluid Assay

  • Mineral content deposition quantification – colorimetric assay technique
  • Mineral content deposition visualization – SEM

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